Journal: BMC Cancer

Article Title: Frameshift mutations in coding repeats of protein tyrosine phosphatase genes in colorectal tumors with microsatellite instability

doi: 10.1186/1471-2407-8-329

Figure Lengend Snippet: Electropherograms of allelic shifts . Electropherograms of shifted alleles. DNA fragment analysis of a representative set of PTP cMNRs in a MSI-H colorectal tumor (KS2966A, Sam49) and MSI-H cancer cell lines (Co115, TC71, and HCT116). Corresponding wildtype alleles (wt, lower panel) are indicated together with tumor cell-specific allelic shifts marked by asterisks (upper panel). Allele intensities were determined (peak area) and ratios (f) of wildtype and novel alleles in primary tumors and cell lines were calculated, defining a 2-fold difference as threshold for allelic shifts.

Article Snippet: Primer design, DNA fragment and data analysis was performed as described [ ].

Techniques:

Journal: Journal of Experimental Botany

Article Title: Induction of vacuolar invertase inhibitor mRNA in potato tubers contributes to cold-induced sweetening resistance and includes spliced hybrid mRNA variants

doi: 10.1093/jxb/err043

Figure Lengend Snippet: INH forms isolated from potato genotypes 1021/1 and 937/3 using a combination of cDNA library screening and PCR amplification from genomic DNA and cDNA.

Article Snippet: PCR amplification using the primer pair, cDNA template, and PCR Extender proofreading Taq DNA polymerase (5 Prime Co., Gaithersburg, MD, USA) resulted in a band of ∼800 bp, which was ligated into pBluescript and six clones sequenced for each cultivar.

Techniques: Isolation, cDNA Library Assay, Amplification